Abstract :

 An asymmetric immobilization process that immobilizes a one or more receptor biomolecules on one or more solid surfaces is provided. The asymmetric immobilization process includes the following steps: 

(i) activating the one or more solid surfaces using an oxidizing agent;

(ii) treating the one or more solid surfaces with aminosilane to obtain a one or more amine functionalized solid surfaces; 

(iii) treating the one or more receptor biomolecules with a 1-Ethyl-3-(3 dimethylaminopropyl)carbodiimide (EDC) to obtain a one or more site-specific EDC activated receptor biomolecules; and 

(iv) immobilizing the one or more site-specific EDC activated receptor biomolecules on a first layer of the one or more amine functionalized solid surfaces, wherein the immobilizing comprises treating the one or more site-specific EDC activated receptor biomolecules with amine groups of the first layer of the one or more amine functionalized solid surfaces to form a covalent amide bond.